Development of TaqMan-based real-time RT-PCR assay based on N gene for the quantitative detection of feline morbillivirus

Abstract Background Morbilliviruses are categorized under the family of Paramyxoviridae and have been associated with severe diseases, such as Peste des petits ruminants, canine distemper measles evidence high morbidity and/or could cause major economic loss in production livestock animals, goats sheep. Feline morbillivirus (FeMV) is one members that has speculated to chronic kidney disease cats even though a definite relationship still unclear. To date, FeMV detected several continents, Asia (Japan, China, Thailand, Malaysia), Europe (Italy, German, Turkey), Africa (South Africa), South North America (Brazil, Unites States). This study aims develop TaqMan real-time RT-PCR (qRT-PCR) assay targeting N gene clinical samples detect early phase infection. Results A specific was developed, since no amplification observed viral strains from same , virus (CDV), Newcastle (NDV), (MeV), other feline viruses, coronavirus (FCoV) leukemia (FeLV). The lower detection limit 1.74 × 10 4 copies/?L Cq value 34.32 ± 0.5 based on cRNA copy number. coefficient variations (CV) values calculated for both intra- inter-assay were low, ranging 0.34–0.53% 1.38–2.03%, respectively. In addition, sample evaluation using this showed higher rate, 25 (35.2%) being FeMV-positive compared 11 (15.5%) conventional RT-PCR, proving more sensitive RT-PCR. Conclusions TaqMan-based described sensitive, specific, rapid, reproducible gene, which be used infection cats.